Mapping of avirulence genes and mating type loci in Sclerospora graminicola, the pearl millet downy mildew pathogen
Duration: 03/Nov/2010 - 02/Nov/2012
Donor: Government department -national- state or local,Department of Biotechnology- Government of India-India
The project aims at developing SSRs and DArT markers for S. graminicola and identification of DNA markers linked to Avr genes and mating type loci. For the development of microsatellite markers, partial genomic library of S. graminicola will be constructed and screened with a mixture of oligonucleotide micosatellite probes. Positive clones will be identified and sequenced to design primers to the regions flanking the SSRs. These SSRs will be selected by analyzing the PCR products of different S. graminicola isolates. The SSRs marker reported in the related oomycetous pathogen Phytophthora would be tested to detect polymorphism in S. graminicola populations. For DArT, the genomic representation will be prepared from the DNA of the parental isolates after subjecting it to a complexity reduction procedure. The genomic representation will be printed on the slide and hybridized with the representation from the parental isolates and the polymorphism between the parents and the population will be scored based on differential hybridization pattern. Mapping populations for mating types will be developed by crossing reference mating type isolates Sg 018 (Mat-1) and Sg 019 (Mat-2). Mating types of F1s will be determined by backcrossing with each parent and examining for oospore production.