Harnessing RNA interference (RNAi) technology for resistance to peanut bud necrosis virus (PBNV) in groundnut
Duration: 28/Jun/2010 - 27/Jun/2013
Donor: Government department -national- state or local,Department of Biotechnology- Government of India-India
CGIAR Research Program: Grain Legumes
RNAi technology has been one of the key biological breakthroughs of the last decade and has revolutionized basic biology, gene function studies, and it also holds promise in disease diagnosis and management. It involves a coordinated series of sub-cellular events that ultimately lead to the termination or reduction of gene expression.
This project aims to develop transgenic groundnut with resistance to the peanut bud necrosis virus (PBNV) through RNAi approaches, thereby improving the productivity and quality of groundnut crop and products. Regions of the PBNV genome that would be best targets for suppression would be selected and used to assemble RNAi constructs in plant expression vectors that will then be used to transform groundnut. Transgenic plants will be evaluated for resistance against PBNV under contained greenhouse conditions following mechanical inoculation with the virus. Transgenic plants of groundnut will be developed with these constructs by using Agrobacterium tumefaciens-based gene transfer systems for stable transformation of groundnut that can produce a high efficiency of transgenic events. An efficient protocol has already been developed at ICRISAT which is routinely used for transforming groundnut with high frequency.