Duration: 01/May/2012 - 30/Apr/2015
Project contact: Sharma,S Upadhyaya, HD
Donor: Science & Engineering Research Board-Government of India-India
Chickpea is the major pulse crop for sustaining farming systems. The development of high yielding varieties with a broad genetic base is a major objective of genetic improvement programs, which in turn depends upon the use of elite and diverse parental lines. The use of conventional breeding methods requires repeated selfing and rigorous selection for several generations in the segregating material for the development of homozygous elite parental lines as well as in varietal development program, which makes these techniques time-consuming, resource-demanding and labor-intensive. The generation of DH will greatly reduce the time required for obtaining homozygous lines if an efficient haploid generation protocol is available. In the present investigation, the anther and microspore culture protocols to generate DH in chickpea will be developed and standardized. Three to five germplasm accessions belonging to cultivated chickpea and wild Cicer species will be used to standardize the protocol for anther and microspore cultures which include microspore isolation, callus induction, haploid regeneration, and diploidization of haploid plants following colchicine’s treatment to generate DH. Once the protocols are standardized for these genotypes, the efficiency of these protocols will be tested on other genotypes and F1 crosses.